B0300

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B0300

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Betaine solution

5 M, PCR Reagent

CAS Number:107-43-7
Linear Formula:(CH3)3N+CH2COO−
Molecular Weight:117.15
Beilstein Registry Number:3537113
MDL number:MFCD00012123
PubChem Substance ID:24891511

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Description

ApplicationThe addition of betaine at a final concentration of 0.8-1.6M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions. The addition of betaine has been reported to enhance the specificity of the polymerase chain reaction by eliminating the base pair composition dependence of DNA melting.1
Legal InformationThe addition of betaine has been reported to enhance the specificity of the polymerase chain reaction by eliminating the base pair composition dependence of DNA melting (Rees, WA et al).

The purchase of this product does not include a license to practice the claims of Patent Nos. US 5,545,539, US 6,270,962, DE4411588, or DE4411594. The practice of the claims of these patents may require a license from the patent owners.

Properties

gradePCR Reagent
packagingvial of 1.5 mL
concentration5 M
foreign activityDNase, RNase, none detected
storage temp.2-8°C

Safety

WGK Germany3

References

Cited Reference1. Rees, W.A., et al., Betaine can eliminate the base pair composition dependence of DNA melting. Biochemistry 32, 137-144, (1993) Abstract
referenceHenke, W., et al., Betaine improves the PCR amplification of GC-rich DNA sequences. Nucleic Acids Res. 25, 3957-3958, (1997) Abstract